![]() ![]() Para evaluar el efecto de los pretratamientos, se cuantificó proteína por el método de Bradford, digestibilidad in vitro, capacidad antioxidante y fenoles totales. El objetivo de este estudio fue aplicar diferentes pretratamientos: enzimático, alcalino, térmico seco y térmico húmedo. Diferentes estudios resaltan que el polen presenta restricción en su absorción de nutrientes por su compleja pared celular externa, siendo poco digerible para especies monogástricas como abejas y seres humanos. Keywords: Cell wall, chemical, enzymatic, hydrolysis, thermal.Įl polen apícola se caracteriza por su alto contenido nutricional que podría ser empleado en la alimentación de los seres humanos, específicamente por su valor en proteína y capacidad antioxidante. Finally, the variables assessed by principal component analysis showed differences for every pretreatment. Such results coincide with microstructural changes observed in pretreated pollen micrographs. In relation to antioxidant capacity, it showed a non-representative decrease regarding other vegetable matrices, with the exception of a raise in phenols for some pretreatments. Protein estimated by Bradford method decreased in pretreated pollen, due to the breakage of peptide bonds, and the digestibility raise from 62% in untreated pollen to 85-98% in pretreated pollen. ![]() In order to evaluate the effect of each pretreatment, protein by Bradford method, in vitro digestibility, antioxidant capacity and total phenols were quantified. The objective of this study was to apply different pretreatments: enzymatic, alkaline, dry thermal and wet thermal. Different studies emphasize that pollen shows a restriction in nutrient absorption caused by its complex external cell wall, being not easily digestible by monogastric species as bees and humans. Bee pollen is characterized by its high nutritional value that could be used in human diet, specifically for its value in protein and antioxidant capacity.
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